ALKBH5 and colorectal carcinoma: To evaluate the function of ALKBH5 in CRC, ALKBH5 was silenced through two shRNA targeting the ALKBH5 (shA5#1, shA5#2), while stably upregulated through a lentivirus vector in HCT116 and SW480 cells according to the ALKBH5 expression in CRC cells (Figure 1C and D).