The results of the Cox univariate analysis for PNI(+) patients showed that preoperative intestinal obstruction, postoperative chemotherapy, vascular tumour thrombus, degree of differentiation, N stage, M stage, preoperative combined with cerebrovascular disease, preoperative CEA level, preoperative CA 19 − 9 level, and preoperative CA125 level were prognostic factors for OS. This evidence concerns the gene MUC16 and intestinal obstruction.