One study supports the use of targeted NGS in the screening of EGFR, KRAS, and BRAF mutations in formalin-fixed, paraffin-embedded tumour tissue compared to RT-PCR, as NGS revealed seven non-synonymous single-nucleotide variations and one insertion-deletion variation in EGFR which was not detectable by the RT-PCR methods [13]. The gene discussed is KRAS; the disease is neoplasm.