ERBB2 and triple-negative breast carcinoma: As an example of such a workflow, a commercially obtained fresh-frozen triple negative breast cancer tissue specimen, pathologist assessed for PR, ER, and Her2 by US BioLab (Rockville, MD) (see Supplementary Material for additional information), was first imaged at 20 μm on a Bruker rapifleX by MSI (negative ion mode with DAN matrix for lipid detection).