The study investigated the impact of hyperthermia and Ibuprofen (at a concentration of 1100 μM) on the expression of genes related to cell proliferation (PCNA), tumor suppression (KLF4), apoptosis (P53, BAX, BCL2), and the Wnt signaling pathway (WNT1 and CTNNB1) using qRT-PCR. Here, KLF4 is linked to neoplasm.