The study investigated the impact of hyperthermia and Ibuprofen (at a concentration of 1100 μM) on the expression of genes related to cell proliferation (PCNA), tumor suppression (KLF4), apoptosis (P53, BAX, BCL2), and the Wnt signaling pathway (WNT1 and CTNNB1) using qRT-PCR. This evidence concerns the gene WNT1 and neoplasm.