We also stimulated the 143B isogenic cell lines with cGAMP and found that the 143B ATRX KO cell line had significantly less expression of IFNB1, suggesting that at least part of the CGAS/STING pathway impairment was occurring downstream of CGAS in our human sarcoma cell lines (Figure 7H). The gene discussed is IFNB1; the disease is sarcoma.