In addition, our RT-qPCR also further demonstrated that EZH2 could regulate the expression of E2F1, GLI1, CDK3 and Mcm4. It is suggested that the regulation of EZH2 in PC may be related to E2F1, GLI1, CDK3 and Mcm4. The gene discussed is E2F1; the disease is pachyonychia congenita.