In conclusion, our study, for the first time, identified the chemical constituents in RSGB by UPLC-QTOF-MS/MS and demonstrate that the improvement of renal fibrosis by RSGB is related to the inhibition of Tgfβ1/Smad2/3 pathway, Wnt4/β-catenin pathway and NGFR/NF-κB pathway. Here, SMAD2 is linked to renal fibrosis.