TBX1 and chordoma: In addition, VLP-packaged Cas9/brachyury gRNA RNP a + c and a + d were more efficient at decreasing brachyury gene and protein expression (Fig. 3b, c and d, and Supplementary Fig S3b) and chordoma cell growth (Fig. 3e and Supplementary Fig S3c) compared to VLP-packaged Cas9/brachyury gRNA RNP b + c and b + d.