Additionally, compared with normal esophageal epithelial cells CP-H031, IL-1RA was markedly downregulated in the human ESCC cell lines KYSE510, KYSE410, Eca109, and TE-1, as shown in Fig. 2A and B. These findings suggest that the lack of IL-1RA may influence the onset and progression of ESCC. This evidence concerns the gene IL1R1 and esophageal squamous cell carcinoma.