NFKB1 and esophageal squamous cell carcinoma: Furthermore, the bioinformatics analysis indicated that IL-1RA could inhibit the PI3K/N-FKB signaling pathway, which was confirmed by western blotting showing that overexpression of IL-1RA can significantly inhibit PI3K expression and phosphorylation of NF-κB in ESCC cells (Fig. 7D).