CTLA4 and melanoma: Similarly, further analysis showed that not those with primary melanoma (stages 0-II, n = 165, p = 0.23) (Figure 2I) but those with metastatic melanoma (stages III-IV, n = 98) had an association between low blood CTLA4 expression and worse OS based on the optimal cut point (log –4.73); however, it was not statistically significant (p = 0.053) (Figure 2J).