Notably, knockdown of TAK1, mediated by AAV-siTAK1, significantly increased RIPK1-caspase 8-mediated apoptosis and RIPK1-RIPK3-MLKL dependent necroptosis of neurons in the cerebral cortex and hippocampus, which was accompanied by the decrease of neurons and cognitive impairment. Here, MLKL is linked to Cognitive impairment.