Immune checkpoint analysis showed that CD44, CD40LG, CD276 and TNFSF18 were significant difference between the two clusters as shown in Fig. 6C. These results suggested that FMR1, LRPPRC, RBMX, YTHDC2 and IGF2BP1 might be involved in the development of CRC by participating in the immune regulation process of CRC. The gene discussed is CD44; the disease is colorectal carcinoma.