However, we found that HUNK suppresses metastasis of CRC cells via activating GEF-H1 by direct phosphorylation at S645 site, which promotes GTP-bound RhoA, thus consequently leading to a sequential phosphorylation event of ROCK/LIMK-1/CFL-1 [11, 13]. The gene discussed is CFL1; the disease is colorectal carcinoma.