In terms of CLTA-4, some preclinical studies testified that knockout of CTLA-4 can also strengthen anti-tumor activity in colon cancer and bladder cancer (Shi et al., 2017; Zhang et al., 2019).Though manipulating immune checkpoints in autologous T cells exhibited enhanced anti-tumor effect to some extent, it only triggered responses in selected patients. Here, CTLA4 is linked to neoplasm.