We then injected the rAAV2/9-hSyn–FSF–FLEX-tdTomato (tdT) together with the rAAV2/9-hSyn-RSR-FLEX-enhanced green fluorescence protein (GFP) infectious virus particles into the MS of AD/TERM mice, resulting in the expression of tdT in D28K+ neurons (D28K+ ​tdT) and GFP in D28K− cholinergic neurons (D28K−GFP), respectively (Fig. S1). Here, CALB1 is linked to Alzheimer disease.