Immunofluorescent staining illustrated that the CD31 (marker of blood vessels) coverage was lower after JP1 treatment (Figure 2, A and B); however, the α-SMA (marker of mural cells), claudin 5 (marker of endothelial cells), and desmin (marker of pericytes) coverage was higher after JP1 treatment in melanoma (Figure 2, C–E). This evidence concerns the gene DES and melanoma.