To validate the role of C/EBP-β in IL-1β-mediated induction of C3 expression by ZIKV infection in THP-1 cells, we infected cells with ZIKV at 5 MOI and analyzed the activation of P38, Erk1/2, and C/EBP-β using Western blotting. The gene discussed is MAPK3; the disease is Zika virus infectious disease.