Western blotting showed that the TET1 expression increased from day 3 and persisted for 21 days after tumor cell inoculation with the progression of the bone tumor (Figure 2D), while the TET2 expression remained unchanged (Figure 2E) and the TRPV4 expression increased from day 7 and persisted for 21 days after tumor cell inoculation (Figure 2F). The gene discussed is TET1; the disease is bone neoplasm.