Immunopositivity for Hsp60 was present in the cytoplasm of the epithelial cells with a granular pattern in benign goiter (BG) (Figure 4A,B), with an average of 5.50 ± 3.07, as well as with a diffuse pattern in Hurthle cell carcinoma (HC) (Figure 4C,D), medullary carcinoma (MC) (Figure 4E,F), and anaplastic carcinoma (AC) (Figure 4G,H), with an average of 73.75 ± 7.38, of 88.37 ± 3.46, and of 88.50 ± 5.97, respectively. This evidence concerns the gene HSPD1 and medullary thyroid gland carcinoma.