We first conducted a cross-dataset analysis of single-cell RNA-sequencing analysis (scRNA-seq) data on the primary tumors and liver metastatic tumors of LSL-KrasG12D/+;Trp53R172H/+;Pdx1-Cre (KPC) mice [7,8] with matched animal background and scRNA-seq methodology (Figure 1A,B). This evidence concerns the gene PDX1 and metastatic neoplasm.