A significantly reduced level of E3 ligase NEDD4L has been found in esophageal squamous cell carcinoma (ESCC) clinical samples; the overexpression of NEDD4L inhibited the cell viability, cell cycle progression, and glutamine metabolism via the ubiquitination of c-Myc to reduce the expression of GLS1 and SLC1A5 [136]. This evidence concerns the gene GLS and esophageal squamous cell carcinoma.