For example, Annexin A6, which is a calcium-dependent membrane-binding protein that is closely associated with several cancers such as melanoma, epithelial carcinoma, cervical, breast and prostate cancer [91], was not identified in our 100 K EVs, but was found mostly in our 10 K EVs and in only one 18 K EVs derived from the MM05 cell line. Here, ANXA6 is linked to prostate cancer.