On the other hand, Gaydosik and colleagues showed, using single-cell RNA sequencing (scRNA seq) in skin biopsies, that CD8+ tumor-infiltrating lymphocytes (TILs) expressed genes encoding for co-inhibitory receptors including PD1, CTLA4, TIME3, LAG3, and TIGIT at variable levels across five advanced-stage CTCL patients [50]. This evidence concerns the gene PDCD1 and primary cutaneous T-cell non-Hodgkin lymphoma.