In another investigation, it has been shown that PGK1 induced the phosphorylation of PRAS40 (the proline-rich AKT substrate of 40 kDa, encoded by the gene AKT1S1) at Thr246 in Ewing’s sarcoma cell line A673, and liver cancer cell lines HepG2 and SNU449. The gene discussed is AKT1S1; the disease is liver cancer.