ALK and non-small cell lung carcinoma: In this study, we demonstrated how, in a clinical setting, a comprehensive multiplexing approach for gene fusion detection that simultaneously queries multiple potentially actionable targets (e.g., ALK, ROS1, RET, NTRK1, NTRK2, NTRK3 and MET ex14skipping) is feasible by targeted NGS using total RNA extracted from FFPE NSCLC clinical samples.