On the other hand, MΦ incubated with L. shawi EVs (after 72 h pLsEV5 = 0.0157, pLsEV10 = 0.0392, pLsEV20 = 0.0206, pLsEV45 = 0.0084), L. shawi Ag (p24h = 0.0046, p48h = 0.0044, p72h = 0.0342), L. guyanensis EVs (after 72 h pLgEV20 = 0.0371, pLgEV45 = 0.006), or L. guyanensis Ag (p24h = 0.046, p48h = 0.0387, p72h = 0.0002) exhibited a reduction in de novo urea production when compared to promastigote infection (Figure 9). This evidence concerns the gene RENBP and infection.