To verify our hypothesis, we performed a wide range of analyses: investigation of the ZWILCH expression profile in tumors with publicly available TCGA datasets (i), analysis of ZWILCH expression with publicly available transcriptomic data from the GEO database (ii), the study of ZWILCH expression in normal adrenals and adrenocortical carcinoma using biological samples (iii), and examination of ZWILCH protein expression in commercially available tissue microarrays, containing adrenal tumors (adrenal cancer progression) and normal adrenal gland tissues regarding Ki67 status and tumor size (iv). Here, MKI67 is linked to adrenal cortex carcinoma.