Moreover, using a phosphoproteomics approach, we previously assessed the enzymatic activity of three enzymes associated with cancer and only present in sEVs derived from the cancerous cell lines MCF7 and MDA-MB-231: ATP citrate lyase (ACLY), sirtuin-1 (SIRT1) and sirtuin-6 (SIRT6) [16]. The gene discussed is SIRT1; the disease is cancer.