The results of this study indicate that mutations in the GLA/Gla gene lead to the accumulation of Gb3/Lyso-Gb3 in central nervous system tissues, triggering the activation of microglial cells and resulting in increased production of pro-inflammatory cytokines, such as IFNγ, TNFα, IL1β, and IL6, in Fabry disease. This evidence concerns the gene TNF and Fabry disease.