The detection of syndecan-4 FL (35–40 kDa) and the CF (10–15 kDa) in diseased human myocardium is shown here for demonstration of the protein bands and the ongoing syndecan-4 shedding observed in heart disease (Figure 1B), with biological variation in human samples, i.e., a stronger syndecan-4 signal in two out of the three samples tested. The gene discussed is SDC4; the disease is heart disorder.