The cut-off point was established based on data from a multicenter study that analyzed plasma CK-18, also with an M30-Apoptosense ELISA kit, in 150 healthy subjects with histologically excluded NASH through liver biopsy and for whom age, sex and BMI were similar to those of our cohort [27]. The gene discussed is KRT18; the disease is metabolic dysfunction-associated steatohepatitis.