The results of the pseudotime analysis of the macrophages further confirmed that signal molecules in the regulatory network are key factors affecting the change in the functional state of macrophages, and the abnormal expression of ligand signals (TIMP1, VEGFA, TGFB1, LIF, CCL3L3, BMP2, SPP1) in tumor cells may be an important reason for the transformation of macrophages into an immunosuppressive state. This evidence concerns the gene LIF and neoplasm.