In this study, the main goal was to assess the feasibility of a CEA-targeted Nanobody conjugated to two zwitterionic dyes, ZW800-Forte and ZW800-1, to facilitate the visualization of pancreatic cancer and characterize the results with respect to tumor specificity, mean fluorescence intensity and tumor-to-background ratio. This evidence concerns the gene CEACAM5 and familial pancreatic carcinoma.