To investigate the impact of bacterial vesicles from the sepsis-associated gram-negative pathogens Escherichia coli (EcOMVs), Klebsiella pneumoniae (KpOMVs) and Salmonella enterica serovar Typhimurium (SalOMVs) as well as the gram-positive pathogen Streptococcus pneumoniae (SpMVs) on endothelial RNase1, OMVs and MVs were isolated from liquid bacterial culture via UF-SEC (Fig. S2A). The gene discussed is RNASE1; the disease is Sepsis.