However, here, while CB1 mRNA tended to increase in the colons of ABX mice, we were unable to detect changes in GF mice, which may be due to the fact that we utilized Balb-c mice here compared to C57BL/6J mice in the work of Manca et al. Additionally, recent studies have shown that the anti-inflammatory effect of some molecules targeting the eCBome (CBD and CBDV) on colon inflammation in mice is linked with alteration of the gut microbiota composition [25, 26]. Here, CNR1 is linked to colitis.