The proliferative capacity of HCC cells overexpressing ANGPTL8 was also inhibited by adding an inhibitor of autophagic flux, indicating that DEN-induced upregulation of ANGPTL8 expression activates the ROS/ERK pathway, which induces autophagy and promotes HCC cell proliferation and hepatocarcinogenesis. This evidence concerns the gene ANGPTL8 and hepatocellular carcinoma.