To examine the effects in vivo, luciferase-labeled MHCC97H cells (MHCC97H-Luc) with ANGPTL8 KD or OE constructs or with nontargeting/empty vectors were orthotopically injected into the livers of nude mice, and tumor development was monitored using bioluminescence imaging. This evidence concerns the gene ANGPTL8 and neoplasm.