Our RNA-seq results also showed that ANGPTL8 can significantly upregulate CD68 expression, suggesting that ANGPTL8 may recruit a large number of macrophages into liver cancer tissues, so we next compared the number of Kupffer cells, which are the resident macrophage cells of the liver, between DEN-induced tumors from WT and ANGPTL8-KO mice. This evidence concerns the gene CD68 and liver cancer.