We therefore speculated that ANGPTL8 may play a role in hepatocarcinogenesis, and results showed that DEN, a known chemical carcinogen that induces hepatic tumors similar to HCC, upregulated ANGPTL8 expression in the livers of mice (Fig. S2E), so we next induced hepatocarcinogenesis by combining DEN plus CCL4 treatment (Fig. 1A). This evidence concerns the gene CCL4 and hepatocellular carcinoma.