After treating osteosarcoma cells with ERIANIN, it was found that the intracellular acid-vesicle organelles increased, the expression of LC3-ll, p62, and Beclin1 was upregulated, and ROS accumulation was induced, which induced an increase in the phosphorylation levels of JNK and c-Jun, suggesting that ERIANIN activated the ROS/JNK pathway to induce autophagy in osteosarcoma cells. The gene discussed is MAPK8; the disease is osteosarcoma.