SPP1 and esophageal cancer: Kaplan-Meier Plotter (https://kmplot.com/analysis/) and Gene Expression Profiling Interactive Analysis 2 (GEPIA2, https://gepia2.cancer-pku.cn/) online database were used to verify the expression of the specific signature genes of the TREM2+ TAM subpopulation, which demonstrated TREM2, SPP1, APOE, C1QC, C1QB, and C1QA were highly expressed in esophageal cancer (Supplementary Figure S2) and correlated with poor prognosis (Supplementary Figure S3).