CD8 Tex with elevated expression of multiple inhibitory receptors (PDCD1, CTLA4, LAG3, TIGIT, HAVCR2) and Tregs with high expression levels of FOXP3, TIGIT, and CTLA4 (Supplementary Figure S1C) were enriched in the ESCC TME (Figure 1F), which contributed to tumor immune escape and immunotherapy resistance (39, 43). The gene discussed is LAG3; the disease is esophageal squamous cell carcinoma.