Kaplan-Meier Plotter (https://kmplot.com/analysis/) and Gene Expression Profiling Interactive Analysis 2 (GEPIA2, https://gepia2.cancer-pku.cn/) online database were used to verify the expression of the specific signature genes of the TREM2+ TAM subpopulation, which demonstrated TREM2, SPP1, APOE, C1QC, C1QB, and C1QA were highly expressed in esophageal cancer (Supplementary Figure S2) and correlated with poor prognosis (Supplementary Figure S3). This evidence concerns the gene C1QA and esophageal cancer.