Developmental trajectories of scRNA-seq have revealed that T helper 17 (Th17) cells are located in the midst of differentiation from naive CD4+ T cells to Tregs, representing a transitional state (30).With the development of lung cancer, the proportion of effector CD4+ T cells gradually decreases, whereas the proportion of exhausted CD4+ T cells considerably increases. This evidence concerns the gene CD4 and lung carcinoma.