To determine whether RV Wt1-5 binds to co-receptor cell surface proteins (Hsp90, Hsp70, Hsp60, Hsp40, Hsc70, integrin β3, or PDI) during the initial events of infection, enriched membrane fractions obtained from gastric subtype-intestinal adenocarcinoma (Ti2) were incubated with RV in ELISA plates with hyperimmune sera against RV as capture antibody, and primary antibodies were used for the detection of each of the cellular proteins evaluated. This evidence concerns the gene HSP90AA1 and infection.