To investigate the involvement of the Hippo pathway in invadopodia formation and function, we subjected the entire panel of the 21 cancer cell lines (both those scored positive and those scored negative for matrix degradation and invadopodia formation) to siRNA-mediated knockdown of YAP, TAZ or both (SMARTpool siRNAs are listed in Table S2). This evidence concerns the gene YAP1 and cancer.