To demonstrate the mechanism of the Wnt pathway and tumor progression, a western blot analysis was performed to demonstrate the protein expression of β-catenin, p-GSK3B ser9, VEGF, Ki-67, and cyclin D1 following GSK3B and galectin-3 knockdown in GBM8401 and U87MG cells (Figure 4). This evidence concerns the gene GSK3B and neoplasm.