Furthermore, a randomized clinical trial involving 117 obese patients with prediabetes showed that the administration of capsules containing 450 mg of Nigella sativa oil twice a day or 500 mg of metformin twice a day led to similar improvements in anthropometric indices, glycemia, lipid profile, and inflammatory parameters (TNF-α (tumor necrosis factor-α) levels decreased significantly) [44]. The gene discussed is TNF; the disease is prediabetes syndrome.