Among the 397 identified DE genes, 56 genes were significantly correlated with CD151, MMP1, PVT1, and SKP2 as reference genes for lymph node metastasis, and due to meeting best criteria in the ROC analysis were known be excellent for the distinguish lymph node metastasis cancer from primary LUSC without metastasis (N0). This evidence concerns the gene SKP2 and metastatic malignant neoplasm in the lymph nodes.