Immunofluorescence analysis indicated that overexpression of KRT7-AS markedly increased PTEN protein levels in SPC-A-1 cells (Fig. 7C), whereas silencing of KRT7-AS notably decreased PTEN protein levels in A549 cells (Fig. 7D); of note, the PTEN mRNA levels were not affected by either overexpression of KRT7-AS or silencing of KRT7-AS in the cancer cells (Fig. S5B, C, E). This evidence concerns the gene PTEN and cancer.