TNF and obesity due to melanocortin 4 receptor deficiency: To investigate the impact of HF diet-induced obesity in early life on systemic (plasma) and mammary inflammation in later life, the mRNA and protein levels of inflammation-related genes, including Tnfα, Il-6, Ccl2, Ccr2, Il-1β, Cox2, Il-13, and Il-4, were measured in plasma, iMT and VAT.