We found that ITF2 inhibition facilitated NF-κB translocation to the nucleus and upregulated the expression of pro-tumorigenic cytokines such as IL-6, TNF, IL-1β, and IL-8; thus, these results led us to hypothesize that chronic inflammation without AOM might induce tumor formation in ITF2 conditional knockout mice. This evidence concerns the gene TCF4 and infectious otitis media.