In the KEGG analysis, the 10 major KEGG signaling pathways are ECM–receptor interaction, Hypertrophic cardiomyopathy, Dilated cardiomyopathy, Rap1 signaling pathway, p53 signaling pathway, Viral protein interaction with cytokine and cytokine receptor, Progesterone-mediated oocyte maturation, Focal adhesion, PI3K-Akt signaling pathway, and Cell cycle. The gene discussed is AKT1; the disease is hypertrophic cardiomyopathy.