The unfused FSHD myocytes demonstrated a higher proportion of DUX4 -ve, DUX4 target gene -ve susceptible (S) cells, while the syncytial FSHD myonuclei demonstrated a greater proportion of DUX4 -ve, DUX4 target gene +ve resigned (R) cells. The gene discussed is DUX4; the disease is facioscapulohumeral muscular dystrophy.